Chrysikopoulos ConstantinosΧρυσικοπουλος ΚωνσταντινοςMaria I. BellouMaria A. TselepiVasiliki I. SyngounaPetros A. KokkinosApostolos VantarakisSpyros C. Paparrodopoulos2024-10-312024-10-3120152015-09-23M. I. Bellou ,V. I. Syngouna , M. A. Tselepi , P. A. Kokkinos , S. C. Paparrodopoulos ,A. Vantarakis , C. V. Chrysikopoulos , "Interaction of human adenoviruses and coliphages with kaolinite and bentonite " ,Scien. of the Tot. Envir.,vol. 517 ,pp.86–95,2015.doi:10.1016/j.scitotenv.2015.02.036https://dspace.library.tuc.gr/handle/123456789/811Human adenoviruses (hAdVs) are pathogenic viruses responsible for public health problems worldwide. They have also been used as viral indicators in environmental systems. Coliphages (e.g., MS2, ΦX174) have also been studied as indicators of viral pollution in fecally contaminated water. Our objective was to evaluate the distribution of three viral fecal indicators (hAdVs, MS2, and ΦΧ174), between two different phyllosilicate clays (kaolinite and bentonite) and the aqueous phase. A series of static and dynamic experiments were conducted under two different temperatures (4, 25 °C) for a time period of seven days. HAdV adsorption was examined in DNase I reaction buffer (pH=7.6, and ionic strength (IS)=1.4 mM), whereas coliphage adsorption in phosphate buffered saline solution (pH=7, IS=2 mM). Moreover, the effect of IS on hAdV adsorption under static conditions was evaluated. The adsorption of hAdVwas assessed by real-time PCR and its infectivitywas tested by cultivation methods. The coliphages MS2 and ΦΧ174 were assayed by the double-layer overlay method. The experimental results have shown that coliphage adsorption onto both kaolinite and bentonite was higher for the dynamic than the static experiments; whereas hAdV adsorption was lower under dynamic conditions. The adsorption of hAdV increased with decreasing temperature, contrary to the results obtained for the coliphages. This study examines the combined effect of temperature, agitation, clay type, and IS on hAdV adsorption onto clays. The results provide useful new information on the effective removal of viral fecal indicators (MS2, ΦX174 and hAdV) from dilute aqueous solutions by adsorption onto kaolinite and bentonite10 pagesenhttp://creativecommons.org/licenses/by/4.0/Peer-Reviewed Journal Publication